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ERX1131998: 454 GS FLX Titanium sequencing
1 LS454 (454 GS FLX Titanium) run: 35,862 spots, 21.8M bases, 45.1Mb downloads

Design: 454 Shotgun
Submitted by: Univerity of Cambridge, DNA Sequencing facility (DNA Sequencing Facility, Department of Biochemistr)
Study: Streptomyces albus DSM41398 genome had been fully sequenced using PacBio and 454 technologies and assembled into a single contig.
show Abstracthide Abstract
Streptomyces albus DSM41398 genome had been sequenced using 454 and PacBio technologies and contains an extra 975Kbp of sequence compared to the published draft of the related strain NBRC 13014. The pacbio assembly had been currated using the 454 data, and ~20 point differences had been found and currated. Also according to pacbio and 454 coverage plots this genome contains nearly 200KB telomeric duplication. According to the antismash v3 it contains 40 secondary metabolite clusters, including salinomycin. Salinomycin has attracted strong renewed interest owing to its potent and selective activity against cancer stem cells and cancer cell lines. Provision of the complete and accurate genome sequence is very important for accurate elucidation of the large PKS typeI multienzyme systems, that are often repetitive and not assembled correctly in the draft genomes.
Sample: Streptomyces albus DSM41398
SAMEA3595485 • ERS902634 • All experiments • All runs
Library:
Name: DSM41398_SG1
Instrument: 454 GS FLX Titanium
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: 454 Titanium V1 shotgun
Runs: 1 run, 35,862 spots, 21.8M bases, 45.1Mb
Run# of Spots# of BasesSizePublished
ERR105171335,86221.8M45.1Mb2016-04-02

ID:
2403262

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